As a consequence individual animals and plant species are threatened with local and then more widespread extinction due to physical barriers, disruption of migration, loss in exchange of genetic material. The overall consequences of habitat fragmentation for wildlife are difficult to assess, as different species respond differently to the loss and isolation of habitat. In general, however, species with large area requirements or strong dependence on a specific type habitat, will be the most vulnerable to habitat fragmentation. With this conference, IENE wants to give experts, decision makers, ecologists, scientists, engineers and professionals the opportunity to exchange ideas, present projects and work, share experiences for a better collaboration and communication. This CD contains all available presentations, abstracts and articles of the conference on Habitat Fragmentation due to transportation infrastructure, held in November 2003 in Brussels.