Agencies can realize a range of tangible benefits from participating in multi-agency collaborative efforts for regional transportation operations. These benefits include access to funding and other resources, improvements in agency operations and productivity, and outcomes that help agencies achieve their mobility and safety goals. This manual uses nine collaborative efforts across the U.S. to illustrate the tangible benefits gained through key strategies such as sharing resources and expertise, performing joint operations, using common operations procedures, and exchanging real-time information. The manual includes a six-step process to allow agencies to estimate their benefits of collaboration.