Incident management is one of the most important functions of a transportation management center (TMC). To efficiently and effectively manage complex regional incidents that affect transportation facilities under the jurisdiction of multiple agencies, TMC operators must be trained appropriately. Recently, a team of universities including the University of Maryland, University of Virginia, and Rensselaer Polytechnic Institute developed a computer-based training tool for regional incident management to be used by the I-95 Corridor Coalition. In its role on the team, the University of Virginia conducted research to support the development of detailed regional incident scenarios to provide content for the computer-based simulation tool. The method created to develop the incident scenarios is presented, with clear examples. Major findings from the research effort include the benefits of rapid prototyping and the usefulness of extensible markup language (XML) for structured content development.