Under the SAFETEA-LU Congressional Bill, the Secretary of Transportation was directed to conduct a national wildlife-vehicle collision (WVC) reduction study. The study was to advance the understanding of the causes and impacts of WVCs and identify solutions to this growing safety problem. A report was submitted to congress in November 2007. This document builds on that report providing a best practices manual for reducing wildlife-vehicle collisions. Design and implementation guidelines are provided for wildlife fencing, wildlife underpasses and overpasses, animal detection systems, vegetation management and wildlife culling. Additionally for a WVC reduction program, information is provided on regional planning, identification of priority areas, alignment and design considerations, guidelines for monitoring effectiveness of mitigations, and potential funding sources.