In order to reduce the climate impact from the transport sector, there is an ambition in the European Union to increase the share of alternative fuels. Since the transport sector is very diverse, there are multiple ways to deal with this. The vehicles can be dedicated towards a certain fuel, or accepting different types of fuel blends where at least part of the fuel consists of conventional types (i.e. diesel or petrol). In recent years, new fuels and blends have been introduced on the market. In Europe, the Commission has presented a road map towards a low carbon economy 2050, a white paper on transport and a proposal for alternative fuel infrastructure. The main focus in these strategies is to reduce the oil dependency and the negative climate impact from the transport sector. In combination with the climate impact, it is important to also involve other aspects of new fuels, such as parameters affecting environment or health. In this study vehicle tests have been performed with five different diesel fuels and the ethanol fuel ED95 used for compression ignited vehicles. Two heavy duty trucks have been used, one diesel truck and one dedicated for ED95, both of EEV emission standard. The tests have been performed on chassis dynamometer, where the vehicles have been driven according to the Worldwide Harmonized Vehicle Cycle (chassis dynamometer version of WHTC).